EMD-0705 Experiments and Validation

Structure of LbCas12a-crRNA complex bound to AcrVA4 (form B complex)

Single particle reconstruction
Overview of EMD-0705
Sample name: LbCas12a-crRNA complex bound to AcrVA4 (form B complex)
Organisms: Lachnospiraceae bacterium, Moraxella bovoculi, synthetic construct
Fitted atomic model: 6klb
Related EM entry by publication: EMD-0704

Map parameters

Recommended contour level: 0.007 (author)
Number of grid points: 400 × 400 × 400
Voxel size: 1.08 × 1.08 × 1.08 Å
Minimum density: -0.036
Maximum density: 0.061
Average density: 0.00
Standard deviation: 0.001

Sample information

Sample name: LbCas12a-crRNA complex bound to AcrVA4 (form B complex)
Ligands: MAGNESIUM ION, water
Nucleic acid: crRNA
Proteins: LbCas12a-crRNA complex bound to AcrVA4 (form B complex), LbCas12a, AcrVA4

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: RELION
Number of particles: 60000
Reconstruction protocol: FOURIER SPACE

Fitting

Initial atomic model: 6KL9
Fitting protocol: flexible
Target criteria: Correlation coefficient
Refinement space: REAL

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 60.0
Energy filter: GIF Bioquantum
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 1.00
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100