EMD-0704 Experiments and Validation

Structure of LbCas12a-crRNA complex bound to AcrVA4 (form A complex)

Single particle reconstruction
Overview of EMD-0704
Sample name: LbCas12a-crRNA complex bound to AcrVA4 (form A complex)
Organisms: Lachnospiraceae bacterium, Moraxella bovoculi, synthetic construct
Fitted atomic model: 6kl9
Related EM entry by publication: EMD-0705

Map parameters

Recommended contour level: 0.016 (author)
Number of grid points: 256 × 256 × 256
Voxel size: 1.08 × 1.08 × 1.08 Å
Minimum density: -0.153
Maximum density: 0.217
Average density: 0.00
Standard deviation: 0.003

Sample information

Sample name: LbCas12a-crRNA complex bound to AcrVA4 (form A complex)
Ligands: MAGNESIUM ION, water
Nucleic acid: RNA (42-MER)
Proteins: LbCas12a-crRNA complex bound to AcrVA4 (form A complex), LbCas12a, AcrVA4

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: RELION
Number of particles: 508000
Reconstruction protocol: FOURIER SPACE

Fitting

Initial atomic model: 5ID6
Fitting protocol: flexible
Target criteria: Correlation coefficient
Refinement space: REAL

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 60.0
Energy filter: GIF Bioquantum
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 1.00
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100