EMD-0695 Experiments and Validation

Cryo-EM structure of human MLL1-NCP complex, binding mode2

Single particle reconstruction
Overview of EMD-0695
Sample name: Human MLL1 complex associated with an unmodified nucleosome, binding mode 2
Organisms: Homo sapiens, Xenopus laevis, synthetic construct
Fitted atomic model: 6kiz
Related EM entries by publication: EMD-0693, EMD-0694, EMD-9998, EMD-9999

Map parameters

Recommended contour level: 0.005 (author)
Number of grid points: 270 × 270 × 270
Voxel size: 1.10 × 1.10 × 1.10 Å
Minimum density: -0.06
Maximum density: 0.10
Average density: -0.00
Standard deviation: 0.004

Sample information

Sample name: Human MLL1 complex associated with an unmodified nucleosome, binding mode 2
Ligands: S-ADENOSYL-L-HOMOCYSTEINE, ZINC ION
Nucleic acids: DNA (145-MER), DNA (145-MER)
Proteins: Human MLL1 complex associated with an unmodified nucleosome, binding mode 2, Human MLL1 complex, unmodified nucleosome, Histone H3, Histone H4, Histone H2A, Histone H2B 1.1, Retinoblastoma-binding protein 5, Histone-lysine N-methyltransferase 2A, WD repeat-containing protein 5, Set1/Ash2 histone methyltransferase complex subunit ASH2

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Number of particles: 31882

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 45.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Vitrification
Cryogen: ETHANE