EMD-0695 Function and Biology

Cryo-EM structure of human MLL1-NCP complex, binding mode2

Single particle reconstruction
4.5Å resolution
Overview of EMD-0695
Sample name: Human MLL1 complex associated with an unmodified nucleosome, binding mode 2
Number of unique components: 15

Human MLL1 complex associated with an unmodified nucleosome, binding mode 2

Protein type
Name: Human MLL1 complex associated with an unmodified nucleosome, binding mode 2

Human MLL1 complex [Homo sapiens]

Protein type
Name: Human MLL1 complex
Expression system
Expression system: Escherichia coli

unmodified nucleosome [Xenopus laevis]

Protein type
Name: unmodified nucleosome

Histone H3 [Xenopus laevis]

Protein type
Name: Histone H3
Theoretical weight (MDa): 0.015
Number of copies: 2
Expression system
Expression system: Escherichia coli

Histone H4 [Xenopus laevis]

Protein type
Name: Histone H4
Theoretical weight (MDa): 0.011
Number of copies: 2
Expression system
Expression system: Escherichia coli

Histone H2A [Xenopus laevis]

Protein type
Name: Histone H2A
Theoretical weight (MDa): 0.014
Number of copies: 2
Expression system
Expression system: Escherichia coli

Histone H2B 1.1 [Xenopus laevis]

Protein type
Name: Histone H2B 1.1
Theoretical weight (MDa): 0.013
Number of copies: 2
Expression system
Expression system: Escherichia coli

DNA (145-MER) [synthetic construct]

Nucleic-acid type
Name: DNA (145-MER)
Theoretical weight (MDa): 0.045
Type: DNA
Structure: OTHER
Synthetic: false
Sequence: (DT)(DC)(DG)(DA)(DG)(DA)(DA)(DT)(DC)(DC)(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG) (DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG)(DA) (DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG)(DC)(DA)(DC)(DC)(DG)(DC)(DT)(DT)(DA)(DA)(DA) (DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC)(DC)(DC)(DC) (DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG)(DG)(DG) (DG)(DA)(DT)(DT)(DA)(DC)(DT)(DC)(DC)(DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA)(DG) (DG)(DC)(DA)(DC)(DG)(DT)(DG)(DT)(DC)(DA)(DG)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DC)(DA) (DT)(DC)(DC)(DG)(DA)

DNA (145-MER) [synthetic construct]

Nucleic-acid type
Name: DNA (145-MER)
Theoretical weight (MDa): 0.045
Type: DNA
Structure: OTHER
Synthetic: false
Sequence: (DT)(DC)(DG)(DG)(DA)(DT)(DG)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DC)(DT)(DG)(DA)(DC)(DA) (DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DC)(DT)(DA)(DG)(DG)(DG)(DA)(DG) (DT)(DA)(DA)(DT)(DC)(DC)(DC)(DC)(DT)(DT)(DG)(DG)(DC)(DG)(DG)(DT)(DT)(DA)(DA)(DA) (DA)(DC)(DG)(DC)(DG)(DG)(DG)(DG)(DG)(DA)(DC)(DA)(DG)(DC)(DG)(DC)(DG)(DT)(DA)(DC) (DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA)(DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA)(DG) (DA)(DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA)(DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)(DA) (DG)(DC)(DG)(DG)(DC)(DC)(DT)(DC)(DG)(DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DT) (DC)(DT)(DC)(DG)(DA)

Retinoblastoma-binding protein 5 [Homo sapiens]

Protein type
Name: Retinoblastoma-binding protein 5
Theoretical weight (MDa): 0.059
Number of copies: 1
Expression system
Expression system: Escherichia coli

Histone-lysine N-methyltransferase 2A [Homo sapiens]

Protein type
Name: Histone-lysine N-methyltransferase 2A
Theoretical weight (MDa): 0.025
Number of copies: 1
Expression system
Expression system: Escherichia coli

WD repeat-containing protein 5 [Homo sapiens]

Protein type
Name: WD repeat-containing protein 5
Theoretical weight (MDa): 0.037
Number of copies: 1
Expression system
Expression system: Escherichia coli

Set1/Ash2 histone methyltransferase complex subunit ASH2 [Homo sapiens]

Protein type
Name: Set1/Ash2 histone methyltransferase complex subunit ASH2
Theoretical weight (MDa): 0.06
Number of copies: 1
Expression system
Expression system: Escherichia coli

S-ADENOSYL-L-HOMOCYSTEINE

Ligand type
Name: S-ADENOSYL-L-HOMOCYSTEINE
Theoretical weight (MDa): 0
Number of copies: 1

ZINC ION

Ligand type
Name: ZINC ION
Theoretical weight (MDa): 0
Number of copies: 1