EMD-0693 Experiments and Validation

Cryo-EM structure of human MLL3-ubNCP complex (4.0 angstrom)

Single particle reconstruction
Overview of EMD-0693
Sample name: Human MLL3 complex associated with an H2B-monoubiquitinated nucleosome (4.0 angstrom)
Organisms: Homo sapiens, Xenopus laevis, synthetic construct
Fitted atomic model: 6kiw
Related EM entries by publication: EMD-0694, EMD-0695, EMD-9998, EMD-9999

Map parameters

Recommended contour level: 0.015 (author)
Number of grid points: 270 × 270 × 270
Voxel size: 1.09 × 1.09 × 1.09 Å
Minimum density: -0.229
Maximum density: 0.336
Average density: -0.00
Standard deviation: 0.012

Sample information

Sample name: Human MLL3 complex associated with an H2B-monoubiquitinated nucleosome (4.0 angstrom)
Ligand: ZINC ION
Nucleic acids: DNA (144-MER), DNA (145-MER)
Proteins: Human MLL3 complex associated with an H2B-monoubiquitinated nucleosome (4.0 angstrom), Human MLL3, H2B-monoubiquitinated nucleosome, Histone H3, Histone H4, Histone H2A, Histone H2B 1.1, Histone-lysine N-methyltransferase 2C, Retinoblastoma-binding protein 5, Ubiquitin, WD repeat-containing protein 5, Set1/Ash2 histone methyltransferase complex subunit ASH2

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Number of particles: 81945

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 40.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Vitrification
Cryogen: ETHANE