EMD-0664

Single-particle
3.03 Å
EMD-0664
3D View Gallery
Deposition: 15/03/2019
Map released: 15/05/2019
Last modified: 18/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-0664
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6o9z summary report (pdf.gz) Map and 6o9z FULL report (pdf.gz)
EMICSS annotations (xml)

Electron cryo-microscopy of the eukaryotic translation initiation factor 2B bound to eukaryotic translation initiation factor 2 from Homo sapiens

EMD-0664

Single-particle
3.03 Å
EMD-0664
3D View Gallery
Deposition: 15/03/2019
Map released: 15/05/2019
Last modified: 18/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Grid
Details: Unspecified
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average electron dose per image: 80.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.03 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 34014
Startup model [1]
Type: OTHER
Details:Negative Stain 3D reconstruction
⦨ Initial angle assignment
Type: OTHER
⦩ Final angle assignment
Type: OTHER
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: EIF2 alpha phosphorylated at serine 51 bound to eIF2B.
⬡ Geometry
X Y Z
Dimensions 352 352 352
Origin 0 0 0
Spacing 352 352 352
Voxel size 0.82199997 Å 0.82199997 Å 0.82199997 Å
Contour list
Primary Level Source
True 1.9 AUTHOR