EMD-0584

Single-particle
3.37 Å
EMD-0584
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Deposition: 17/02/2019
Map released: 10/07/2019
Last modified: 20/03/2024
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-0584
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
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EMICSS annotations (xml)

Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium

EMD-0584

Single-particle
3.37 Å
EMD-0584
3D View Gallery
Deposition: 17/02/2019
Map released: 10/07/2019
Last modified: 20/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Name: Cas9-sgRNA-DNA ternary complex
Complex [1]
Name: Cas9-sgRNA-DNA ternary complex
Natural source [1]
Organism: Streptococcus pyogenes
Protein [1]
LEVO
Name: CRISPR-associated endonuclease Cas9/Csn1
EC number: 3.1.-.-
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [8]
Biological process:
maintenance of CRISPR repeat elements defense response to virus
Molecular function:
DNA endonuclease activity 3'-5' exonuclease activity DNA binding metal ion binding endodeoxyribonuclease activity RNA binding
Natural source
Organism: Streptococcus pyogenes
Molecular weight
Experimental Theoretical Method
- 158 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
RNA [1]
Name: single guide RNA
Number of copies: 1
Natural source
Organism: synthetic construct
Molecular weight
Experimental Theoretical Method
- 33 kDa -
DNA [3]
DNA
Name: 5' product of target strand DNA
Number of copies: 1
Natural source
Organism: synthetic construct
Molecular weight
Experimental Theoretical Method
- 3 kDa -
DNA
Name: non-target strand DNA
Number of copies: 1
Natural source
Organism: synthetic construct
Molecular weight
Experimental Theoretical Method
- 12 kDa -
DNA
Name: 3' product of target strand DNA
Number of copies: 1
Natural source
Organism: synthetic construct
Molecular weight
Experimental Theoretical Method
- 8 kDa -