EMD-0541 Function and Biology

Structure of a MAPK pathway complex

Single particle reconstruction
4.1Å resolution
Overview of EMD-0541
Sample name: Ternary complex of BRAF/MEK1/14-3-3 with MEK inhibitor
Number of unique components: 9
Details: 14-3-3 is heterodimer of Insect epsilon and zeta. model was generated by Insect zeta sequence as a homo-dimer.

Ternary complex of BRAF/MEK1/14-3-3 with MEK inhibitor [Homo sapiens]

Protein type
Name: Ternary complex of BRAF/MEK1/14-3-3 with MEK inhibitor
Experimental weight (MDa): 0.19

Serine/threonine-protein kinase B-raf [Homo sapiens]

Protein type
Name: Serine/threonine-protein kinase B-raf
Theoretical weight (MDa): 0.089
Number of copies: 1
Expression system
Expression system: Spodoptera frugiperda

Dual specificity mitogen-activated protein kinase kinase 1 [Homo sapiens]

Protein type
Name: Dual specificity mitogen-activated protein kinase kinase 1
Theoretical weight (MDa): 0.046
Number of copies: 1
Expression system
Expression system: Spodoptera frugiperda

14-3-3 protein zeta [Beet armyworm]

Protein type
Name: 14-3-3 protein zeta
Theoretical weight (MDa): 0.028
Number of copies: 2

PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER

Ligand type
Name: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER
Theoretical weight (MDa): 0.001
Number of copies: 1

ZINC ION

Ligand type
Name: ZINC ION
Theoretical weight (MDa): 0
Number of copies: 2

ADENOSINE-5'-DIPHOSPHATE

Ligand type
Name: ADENOSINE-5'-DIPHOSPHATE
Theoretical weight (MDa): 0
Number of copies: 1

MAGNESIUM ION

Ligand type
Name: MAGNESIUM ION
Theoretical weight (MDa): 0
Number of copies: 1

5-[(2-fluoro-4-iodophenyl)amino]-N-(2-hydroxyethoxy)imidazo[1,5-a]pyridine-6-carboxamide

Ligand type
Name: 5-[(2-fluoro-4-iodophenyl)amino]-N-(2-hydroxyethoxy)imidazo[1,5-a]pyridine-6-carboxamide
Theoretical weight (MDa): 0
Number of copies: 1