EMD-0497 Experiments and Validation

Cryo-EM reconstruction of CFA/I pili

Helical reconstruction
Overview of EMD-0497
Sample name: Enterotoxigenic Escherichia coli (ETEC) CFA/I pili
Organisms: Escherichia coli ETEC H10407, ESCHERICHIA COLI ETEC O78:H12
Fitted atomic model: 6nrv

Map parameters

Recommended contour level: 0.367 (author)
Number of grid points: 128 × 128 × 128
Voxel size: 1.09 × 1.09 × 1.09 Å
Minimum density: -0.284
Maximum density: 0.807
Average density: 0.033
Standard deviation: 0.108

Sample information

Sample name: Enterotoxigenic Escherichia coli (ETEC) CFA/I pili
Proteins: Enterotoxigenic Escherichia coli (ETEC) CFA/I pili, CFA/I fimbrial subunit B

Validation information

Experimental information

 

Image processing

Fitting

Initial atomic model: 3F85

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 20.0
Holder model: OTHER

Specimen

Preparation
Specimen state: filament
Aggregation parameters (helical parameters)
Phi increment (degrees): 113.3
Azimuthal (Z) increment (Å): 8.5999999999999996
Rotational symmetry: C1
Vitrification
Cryogen: ETHANE
Humidity: 100