EMD-0455

Single-particle
17.0 Å
EMD-0455
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Deposition: 12/01/2019
Map released: 10/07/2019
Last modified: 06/09/2023
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-0455
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EMICSS annotations (xml)

Negative stain EM structure of T. thermophilus Csm with a transcription elongation complex

EMD-0455

Single-particle
17.0 Å
EMD-0455
3D View Gallery
Deposition: 12/01/2019
Map released: 10/07/2019
Last modified: 06/09/2023
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.9
Staining
Type: NEGATIVE
Material: UA
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TECNAI 12
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
Image Recording [1]
Detector model: FEI CETA (4k x 4k)
Average electron dose per image: 58.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 17.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 8351
Startup model [1]
Type: NONE
⦨ Initial angle assignment
Type: ANGULAR RECONSTITUTION
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Csm-RNAP
⬡ Geometry
X Y Z
Dimensions 128 128 128
Origin -64 -64 -64
Spacing 128 128 128
Voxel size 4.36 Å 4.36 Å 4.36 Å
Contour list
Primary Level Source
True 0.03 AUTHOR