EMD-0447

Single-particle
3.49 Å
EMD-0447
3D View Gallery
Deposition: 10/01/2019
Map released: 12/06/2019
Last modified: 20/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-0447
Download
3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6nmd summary report (pdf.gz) Map and 6nmd FULL report (pdf.gz)
EMICSS annotations (xml)

cryo-EM Structure of the LbCas12a-crRNA-AcrVA1 complex

EMD-0447

Single-particle
3.49 Å
EMD-0447
3D View Gallery
Deposition: 10/01/2019
Map released: 12/06/2019
Last modified: 20/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Name: protein complex 2
Complex [1]
Name: protein complex 2
Natural source [1]
Organism: Lachnospiraceae bacterium ND2006
Protein [2]
LEVO
Name: Cpf1
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Lachnospiraceae bacterium ND2006
Molecular weight
Experimental Theoretical Method
- 143 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
LEVO
Name: AcrVA1
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Moraxella bovoculi
Molecular weight
Experimental Theoretical Method
- 20 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - - -
RNA [1]
Name: crRNA
Number of copies: 1
Natural source
Organism: Lachnospiraceae bacterium ND2006
Molecular weight
Experimental Theoretical Method
- 12 kDa -
Ligand [2]
Name: MAGNESIUM ION
HET code: MG
Number of copies: 2
ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 24 Da -
Name: water
HET code: HOH
Number of copies: 6
ChEMBL ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 18 Da -