EMD-0322

Single-particle
3.2 Å
EMD-0322
3D View Gallery
Deposition: 26/10/2018
Map released: 05/12/2018
Last modified: 23/01/2019
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EMD-0322
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6i0y summary report (pdf.gz) Map and 6i0y FULL report (pdf.gz)
EMICSS annotations (xml)

TnaC-stalled ribosome complex with the titin I27 domain folding close to the ribosomal exit tunnel

EMD-0322

Single-particle
3.2 Å
EMD-0322
3D View Gallery
Deposition: 26/10/2018
Map released: 05/12/2018
Last modified: 23/01/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Grid
Model: Quantifoil R2/2
Material: COPPER
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277.15 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 70.0 µm
Nominal CS: 2.7 mm
Nominal defocus: 1.0 µm - 3.0 µm
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: GATAN K2 QUANTUM (4k x 4k)
Number of grids: 1
Number of real images: 2613
Average electron dose per image: 0.926 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.2 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 301510
Software [1]
Name Version Details
RELION 2.1 -
Startup model [1]
Type: PDB ENTRY
PDB model: 4UY8
⦨ Initial angle assignment
Type: PROJECTION MATCHING
⦩ Final angle assignment
Type: PROJECTION MATCHING
Particle selection [1]
Selected Ref. model Method Software Details
468015 - - - -
CTF correction
Software [1]
Name Version Details
Gctf - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Titin I27 domain folded at the exit of ribosomal tunnel
⬡ Geometry
X Y Z
Dimensions 372 372 372
Origin 0 0 0
Spacing 372 372 372
Voxel size 1.06 Å 1.06 Å 1.06 Å
Contour list
Primary Level Source
True 0.019 AUTHOR