EMD-0305
Bacteriophage phi6 dsRNA genome, layer 1, conformation pseudo D3', sub-conformation 2
EMD-0305
Single-particle10.2 Å
Deposition: 18/10/2018
Map released: 12/06/2019
Last modified: 26/06/2019
Buffer
pH: 7.2
Vitrification
Cryogen name: ETHANE
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording
[1]
Final
reconstruction
Resolution: 10.2
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 7015
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 7015
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
RELION | 2.0.3 | Custom version to relax symmetry multi-modal priors |
⦨ Initial angle
assignment
Type:
PROJECTION MATCHING
⦩ Final angle assignment
Type:
PROJECTION MATCHING
CTF correction
Software
[1]
Name | Version | Details |
---|---|---|
RELION | - | - |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: None
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: None
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 512 | 512 | 512 |
Origin | 0 | 0 | 0 |
Spacing | 512 | 512 | 512 |
Voxel size | 1.42 Å | 1.42 Å | 1.42 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.02 | AUTHOR |