EMD-0122 Experiments and Validation

Cryo-EM structure of the hub of the 32 triskelia sweet potato clathrin coat complex

Single particle reconstruction
Overview of EMD-0122
Sample name: Assembly of clathrin heavy and light chain into coat complexes
Organism: Sus scrofa

Map parameters

Recommended contour level: 0.108 (author)
Number of grid points: 256 × 256 × 256
Voxel size: 1.71 × 1.71 × 1.71 Å
Minimum density: -0.069
Maximum density: 0.185
Average density: 0.002
Standard deviation: 0.012

Sample information

Sample name: Assembly of clathrin heavy and light chain into coat complexes
Protein: Assembly of clathrin heavy and light chain into coat complexes

Validation information

Experimental information

 

Image processing

Applied symmetry: C3
Software: RELION
Number of particles: 56352
Reconstruction protocol: BACK PROJECTION

Fitting

Fitting protocol: flexible
Target criteria: Cross-correlation coefficient
Refinement space: REAL

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 53.0
Nominal CS (mm): 2.70
Defocus max (nm): 3.20
Defocus min (nm): 1.40
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 6.32
Vitrification
Apparatus: HOMEMADE PLUNGER
Cryogen: OTHER
Details: 3 uL applied to a grid at room temperature and humidity. 3 second hand blot and plunge..