EMD-0115 Experiments and Validation

Cryo-EM structure of the 32 triskelia sweet potato clathrin coat complex

Single particle reconstruction
Overview of EMD-0115
Sample name: Assembly of clathrin heavy and light chain into coat complexes
Organisms: Sus scrofa, pig

Map parameters

Recommended contour level: 0.04 (author)
Number of grid points: 500 × 500 × 500
Voxel size: 1.71 × 1.71 × 1.71 Å
Minimum density: -0.016
Maximum density: 0.093
Average density: 0.002
Standard deviation: 0.01

Sample information

Sample name: Assembly of clathrin heavy and light chain into coat complexes
Proteins: Assembly of clathrin heavy and light chain into coat complexes, clathrin heavy chain, clathrin light chain b, clathrin light chain a

Validation information

Experimental information

 

Image processing

Applied symmetry: D3
Software: RELION
Number of particles: 1761
Reconstruction protocol: BACK PROJECTION

Fitting

Fitting protocol: flexible
Target criteria: Cross-correlation coefficient
Refinement space: REAL

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 53.0
Nominal CS (mm): 2.70
Defocus max (nm): 3,200.00
Defocus min (nm): 1,400.00
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 6.32
Vitrification
Apparatus: HOMEMADE PLUNGER
Cryogen: OTHER
Details: 3 uL applied to a grid at room temperature and humidity. 3 second hand blot and plunge..