EMD-0096

Single-particle
4.4 Å
EMD-0096
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Deposition: 01/07/2018
Map released: 05/12/2018
Last modified: 11/12/2019
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-0096
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6gys summary report (pdf.gz) Map and 6gys FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of the CBF3-CEN3 complex of the budding yeast kinetochore

EMD-0096

Single-particle
4.4 Å
EMD-0096
3D View Gallery
Deposition: 01/07/2018
Map released: 05/12/2018
Last modified: 11/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: FEI FALCON III (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 27.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.4 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 22668
Startup model [1]
Type: OTHER
Details:Initial model was from SIMPLE_PRIME reconstruction.
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: CBF3-CEN3
⬡ Geometry
X Y Z
Dimensions 400 400 400
Origin 0 0 0
Spacing 400 400 400
Voxel size 1.06 Å 1.06 Å 1.06 Å
Contour list
Primary Level Source
True 0.03 AUTHOR