EMD-0070
Structure of the type IV pilus from enterohemorrhagic Escherichia coli (EHEC)
EMD-0070
Helical reconstruction8.0 Å
Deposition: 20/06/2018
Map released: 15/05/2019
Last modified: 18/12/2019
Buffer
pH: 7.4
Grid
Vitrification
Cryogen name: ETHANE
Chamber humidity: 95%
Chamber temperature: 297 K
Instrument: FEI VITROBOT MARK IV
Chamber humidity: 95%
Chamber temperature: 297 K
Instrument: FEI VITROBOT MARK IV
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording
[1]
Detector model:
FEI FALCON III (4k x 4k)
Detector mode: INTEGRATING
Number of grids: 1
Average exposure time: 2.0 s
Average electron dose per image: 1.4 e/Å2
Detector mode: INTEGRATING
Number of grids: 1
Average exposure time: 2.0 s
Average electron dose per image: 1.4 e/Å2
Final
reconstruction
Resolution: 8.0
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 25669
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 25669
⌯ Applied Symmetry
Software
[1]
Name | Version | Details |
---|---|---|
SPIDER | - | - |
Startup model
[1]
⦩ Final angle assignment
Type:
NOT APPLICABLE
CTF correction
Software
[1]
Name | Version | Details |
---|---|---|
CTFFIND | 3 | - |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 192 | 192 | 192 |
Origin | -96 | -96 | -96 |
Spacing | 192 | 192 | 192 |
Voxel size | 1.09 Å | 1.09 Å | 1.09 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.282 | AUTHOR |