EMD-0070

Helical reconstruction
8.0 Å
EMD-0070 Deposition: 20/06/2018
Map released: 15/05/2019
Last modified: 18/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-0070

Structure of the type IV pilus from enterohemorrhagic Escherichia coli (EHEC)

EMD-0070

Helical reconstruction
8.0 Å
EMD-0070 Deposition: 20/06/2018
Map released: 15/05/2019
Last modified: 18/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Helical reconstruction
Aggregation State: Filament
Specimen preparation [1]
Buffer
pH: 7.4
Grid
Mesh: 300
Material: COPPER
Pretreatment
Type: PLASMA CLEANING
Atmosphere:
Support Film [1]
Material Topology Thickness
CARBON LACEY -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 95%
Chamber temperature: 297 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: FEI FALCON III (4k x 4k)
Detector mode: INTEGRATING
Number of grids: 1
Average exposure time: 2.0 s
Average electron dose per image: 1.4 e/Å2
Image processing [1]
Final reconstruction
Resolution: 8.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 25669
Applied Symmetry
ΔΖ: 11.2 Å
ΔΦ: 96°
Axial symmetry: C1
Software [1]
Name Version Details
SPIDER - -
Startup model [1]
Type: OTHER
Details:Featureless cylinder
Final angle assignment
CTF correction
Software [1]
Name Version Details
CTFFIND 3 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Geometry
X Y Z
Dimensions 192 192 192
Origin -96 -96 -96
Spacing 192 192 192
Voxel size 1.09 Å 1.09 Å 1.09 Å
Contour list
Primary Level Source
True 0.282 AUTHOR