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Indicate the type of the following macromolecular component. If there is more than one unique component (i.e two different proteins etc), please replicate this section and input each unique component separately.
For chimeric proteins which arise from two different sources please replicate the compound and source section and provide the individual sources separately.
Name and describe each macromolecule. Molecules bound to the protein or nucleic acid which are heterogens should be described in the HET section.
For Example: lysyl-tRNA synthetase
A list of known molecules in the UniProt Database can be found here
Give the chain identifier(s) of the given molecule. For example, CHAIN: A, B. If the named molecule is one piece of a single chain, then the chain and the residues which comprise this molecule should be listed. For example: CHAIN: A (47 - 221).
For Example: H, L
Specify a particular domain or fragment of the molecule. For example, SRC domain, or residues 33-78. The domain or fragment may be described by its common name, or as a range of residues, whichever is appropriate. Fragment names are sometimes needed in order to fully describe a molecule, as when a certain sequence of residues has been replaced by a sequence from another source.
For Example: anticodon-binding domain, or none
The Enzyme Commission number associated with this protein, see Enzyme nomenclature database
For Example: 220.127.116.11
Describe any mutations present in the molecule. These may include inserted or deleted residues, substitution mutations, etc. Mutations will be thoroughly described in the SEQUENCE section.
For Example: K56A substitution,2 residues (GLY SER) inserted at the N-terminus., OMP+ mutant
Give any other details that are useful to describe the molecular contents of this entry.
For Example: phosphorothioate link between A 7 and T 8
The scientific name is the Latin genus and species. Virus names such as the Human Immunodeficiency Virus are listed as the scientific name. Names are checked against the NCBI Taxonomy Database
For designed sequences with no biological basis, this field may be satisfied by entering 'NONE'.
For Example: Escherichia coli, or Saccharomyces cerevisiae or none for synthetic
A list of some common organisms can be found here
Common and scientific names are checked against the NCBI Taxonomy Database
For Example: HUMAN, Baker's yeast
Give the strain of the organism.
For Example: gm3c2
Give the name of the variant.
For Example: BRU isolate
Name the specific line of cells from which this component has been derived.
For Example: 4-4-20 murine-murine hybridoma
Identify the collection (DSM, ATCC, NCTC, etc.) and number of the source organism.
DSM - Deutsche Sammlung von Mikroorganismen
ATCC - American Type Culture Collection
NCTC - National Collection of Type Cultures, located at Central Public Health Laboratory, London U.K.
For Example: ATCC 27355
Organized group of tissues that carries on a specialized function.
For Example: heart
Organized group of cells with a common function and structure.
For Example: muscle
Identify the particular kind of cell.
For Example: B-lymphocyte
Give any other remarks or comments on the biological source, expression system or synthesis of the molecule which may be relevant.
If the molecule was isolated from an otherwise unclassifiable substance, list it here. This might include, venom, urine, latex, egg white, etc.
For Example: German collection of microorganisms (DSM)
Was the molecule chemically synthesized?
If the molecule was prepared by purely chemical synthetic methods, ENGINEERED will appear in the COMPND record. The token SYNTHETIC will appear in the SOURCE record, followed by the value YES or an optional remark such as NON-BIOLOGICAL SOURCE.
In the case of a chemically-synthesized molecule using a biologically functional sequence (nucleic or amino acid), the SOURCE will contain the token SYNTHETIC and will also contain the biological origin of the sequence.
This token does not apply to use of a synthetic gene that is expressed biologically.
Was the molecule made using recombinant techniques?
If the molecule was made using recombinant techniques, please describe the expression system. ENGINEERED will appear in the COMPND, followed by the value 'yes' or an optional remark.
If the molecule was made from a synthetic gene, 'ENGINEERED' will appear in the COMPND, and the expression system will be described in SOURCE.
Viruses that are expressed but NOT engineered will have expression systems in the SOURCE records but will NOT have 'ENGINEERED in the COMPND.
Entries containing molecules prepared by recombinant techniques are described as follows:
Was the molecule purified from an organism?
Choose this option if the molecule was purified from the organism mentioned in the relative source section without recourse to expression or synthetic techniques.
Give the scientific name of the expression system used to express the protein or to grow the virus.
Transgenic organisms, such as mouse producing human proteins, are treated as expression systems.
Identify the strain of the expression system.
Identify the variant of the expression system.
Identify the cell line of the expression system.
Name the vector used, i.e., Baculovirus, pzr268.
Identify the plasmid containing the gene.
In this section you may provide any other additional detail regarding the expression, synthesis or purification of the molecule.
For Example: The molecule was synthesized using Edman degradation method