› 7v5g

Electron Microscopy
4.47Å resolution

20S+monoUb-CyclinB1-NT (S1)

Released:
DOI: 10.2210/pdb7v5g/pdb
PDB entry 7v5g coloured by chain, front view.
PDB entry 7v5g coloured by chain, side view.
PDB entry 7v5g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The 20S as a stand-alone proteasome in cells can degrade the ubiquitin tag.
Sahu I, Mali SM, Sulkshane P, Xu C, Rozenberg A, Morag R, Sahoo MP, Singh SK, Ding Z, Wang Y, Day S, Cong Y, Kleifeld O, Brik A, Glickman MH
Nat Commun 12 6173 (2021)
PMID: 34702852
Related structures: EMD-31724

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127173 (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
Proteasome subunit beta type-6 Chains: A, H
Molecule details ›
Chains: A, H
Length: 205 amino acids
Theoretical weight: 21.92 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28072 (Residues: 35-239; Coverage: 86%)
Gene names: LMPY, PSMB6, Y
Sequence domains: Proteasome subunit
Proteasome subunit beta type-7 Chains: B, I
Molecule details ›
Chains: B, I
Length: 234 amino acids
Theoretical weight: 25.32 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q99436 (Residues: 44-277; Coverage: 85%)
Gene names: PSMB7, Z
Sequence domains:
Proteasome subunit beta type-3 Chains: C, J
Molecule details ›
Chains: C, J
Length: 205 amino acids
Theoretical weight: 22.97 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49720 (Residues: 1-205; Coverage: 100%)
Gene name: PSMB3
Sequence domains: Proteasome subunit
Proteasome subunit beta type-2 Chains: D, K
Molecule details ›
Chains: D, K
Length: 201 amino acids
Theoretical weight: 22.86 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49721 (Residues: 1-201; Coverage: 100%)
Gene name: PSMB2
Sequence domains: Proteasome subunit
Proteasome subunit beta type-5 Chains: E, L
Molecule details ›
Chains: E, L
Length: 204 amino acids
Theoretical weight: 22.48 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28074 (Residues: 60-263; Coverage: 78%)
Gene names: LMPX, MB1, PSMB5, X
Sequence domains: Proteasome subunit
Proteasome subunit beta type-1 Chains: F, M
Molecule details ›
Chains: F, M
Length: 213 amino acids
Theoretical weight: 23.58 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P20618 (Residues: 29-241; Coverage: 88%)
Gene names: PSC5, PSMB1
Sequence domains: Proteasome subunit
Proteasome subunit beta type-4 Chains: G, N
Molecule details ›
Chains: G, N
Length: 219 amino acids
Theoretical weight: 24.41 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28070 (Residues: 46-264; Coverage: 83%)
Gene names: PROS26, PSMB4
Sequence domains: Proteasome subunit
Proteasome subunit alpha type-6 Chains: O, V
Molecule details ›
Chains: O, V
Length: 246 amino acids
Theoretical weight: 27.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60900 (Residues: 1-246; Coverage: 100%)
Gene names: PROS27, PSMA6
Sequence domains:
Proteasome subunit alpha type-2 Chains: P, W
Molecule details ›
Chains: P, W
Length: 234 amino acids
Theoretical weight: 25.93 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25787 (Residues: 1-234; Coverage: 100%)
Gene names: HC3, PSC3, PSMA2
Sequence domains:
Proteasome subunit alpha type-4 Chains: Q, X
Molecule details ›
Chains: Q, X
Length: 261 amino acids
Theoretical weight: 29.53 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25789 (Residues: 1-261; Coverage: 100%)
Gene names: HC9, PSC9, PSMA4
Sequence domains:
Proteasome subunit alpha type-7 Chains: R, Y
Molecule details ›
Chains: R, Y
Length: 248 amino acids
Theoretical weight: 27.93 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O14818 (Residues: 1-248; Coverage: 100%)
Gene names: HSPC, PSMA7
Sequence domains:
Proteasome subunit alpha type-5 Chains: S, Z
Molecule details ›
Chains: S, Z
Length: 241 amino acids
Theoretical weight: 26.44 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28066 (Residues: 1-241; Coverage: 100%)
Gene name: PSMA5
Sequence domains:
Proteasome subunit alpha type-1 Chains: T, a
Molecule details ›
Chains: T, a
Length: 263 amino acids
Theoretical weight: 29.6 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25786 (Residues: 1-263; Coverage: 100%)
Gene names: HC2, NU, PROS30, PSC2, PSMA1
Sequence domains:
Proteasome subunit alpha type-3 Chains: U, b
Molecule details ›
Chains: U, b
Length: 255 amino acids
Theoretical weight: 28.47 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25788 (Residues: 1-255; Coverage: 100%)
Gene names: HC8, PSC8, PSMA3
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.47Å
Relevant EMDB volumes: EMD-31724

Quick links

Citations

7 review citations

Localized Proteasomal Degradation: From the Nucleus to Cell Periphery.
Guo X. (2022)
6 more