› 7rg6

X-ray diffraction
2.1Å resolution

Importin alpha2 in complex with ORF4B Bat coronavirus HKU5

Released:
DOI: 10.2210/pdb7rg6/pdb
PDB entry 7rg6 coloured by chain, front view.
PDB entry 7rg6 coloured by chain, side view.
PDB entry 7rg6 coloured by chain, top view.
Source organisms:
Primary publication:
MERS-CoV ORF4b employs an unusual binding mechanism to target IMPα and block innate immunity.
Munasinghe TS, Edwards MR, Tsimbalyuk S, Vogel OA, Smith KM, Stewart M, Foster JK, Bosence LA, Aragão D, Roby JA, Basler CF, Forwood JK
Nat Commun 13 1604 (2022)
PMID: 35338144

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107106 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 510 amino acids
Theoretical weight: 55.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52293 (Residues: 70-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:
Non-structural protein ORF4b Chains: C, D
Molecule details ›
Chains: C, D
Length: 20 amino acids
Theoretical weight: 2.71 KDa
Source organism: Pipistrellus bat coronavirus HKU5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3EXD3 (Residues: 29-48; Coverage: 8%)
Gene name: ORF4b

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 78.692Å b: 90.31Å c: 100Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.205 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Contribution to pathogenesis of accessory proteins of deadly human coronaviruses.
Hurtado-Tamayo et al. (2023)
1 more