› 7m6t

X-ray diffraction
3.19Å resolution

Crystal structure of SOCS2/ElonginB/ElonginC bound to a non-canonical peptide that enhances phospho-peptide binding

Released:
DOI: 10.2210/pdb7m6t/pdb
PDB entry 7m6t coloured by chain, front view.
PDB entry 7m6t coloured by chain, side view.
PDB entry 7m6t coloured by chain, top view.
Source organisms:
Primary publication:
Discovery of an exosite on the SOCS2-SH2 domain that enhances SH2 binding to phosphorylated ligands.
Linossi EM, Li K, Veggiani G, Tan C, Dehkhoda F, Hockings C, Calleja DJ, Keating N, Feltham R, Brooks AJ, Li SS, Sidhu SS, Babon JJ, Kershaw NJ, Nicholson SE
Nat Commun 12 7032 (2021)
PMID: 34857742

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-126989 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Suppressor of cytokine signaling 2 Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 19.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14508 (Residues: 32-198; Coverage: 84%)
Gene names: CIS2, SOCS2, SSI2, STATI2
Sequence domains:
Elongin-B Chain: B
Molecule details ›
Chain: B
Length: 118 amino acids
Theoretical weight: 13.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15370 (Residues: 1-118; Coverage: 100%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family
Elongin-C Chain: C
Molecule details ›
Chain: C
Length: 96 amino acids
Theoretical weight: 10.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15369 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Non-canonical peptide F3 Chain: D
Molecule details ›
Chain: D
Length: 12 amino acids
Theoretical weight: 1.48 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand CAS 2 x CAS

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2221
Unit cell:
a: 60.749Å b: 110.612Å c: 139.896Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.227 0.267
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Evolving cognition of the JAK-STAT signaling pathway: autoimmune disorders and cancer.
Xue et al. (2023)
1 more