› 7m2g

X-ray diffraction
1.79Å resolution

INTERLEUKIN-2 (human) mutant P65K, C125S

Released:
DOI: 10.2210/pdb7m2g/pdb
PDB entry 7m2g coloured by chain, front view.
PDB entry 7m2g coloured by chain, side view.
PDB entry 7m2g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An engineered IL-2 reprogrammed for anti-tumor therapy using a semi-synthetic organism.
Ptacin JL, Caffaro CE, Ma L, San Jose Gall KM, Aerni HR, Acuff NV, Herman RW, Pavlova Y, Pena MJ, Chen DB, Koriazova LK, Shawver LK, Joseph IB, Milla ME
Nat Commun 12 4785 (2021)
PMID: 34373459

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158042 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Interleukin-2 Chain: A
Molecule details ›
Chain: A
Length: 133 amino acids
Theoretical weight: 15.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60568 (Residues: 21-153; Coverage: 100%)
Gene name: IL2
Sequence domains: Interleukin 2

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P43212
Unit cell:
a: 73.501Å b: 73.501Å c: 76.965Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.192 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Emerging principles of cytokine pharmacology and therapeutics.
Saxton et al. (2023)
19 more

1 mention without citation

O-glycosylation and its role in therapeutic proteins.
Thompson et al. (2022)