› 7li8

Electron Microscopy
3.9Å resolution

apo serotonin transporter reconstituted in lipid nanodisc in presence of NaCl in inward open conformation

Released:
DOI: 10.2210/pdb7li8/pdb
PDB entry 7li8 coloured by chain, front view.
PDB entry 7li8 coloured by chain, side view.
PDB entry 7li8 coloured by chain, top view.
Source organisms:
Primary publication:
Illumination of serotonin transporter mechanism and role of the allosteric site.
Yang D, Gouaux E
Sci Adv 7 eabl3857 (2021)
PMID: 34851672
Related structures: EMD-23363

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211046 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Sodium-dependent serotonin transporter Chain: A
Molecule details ›
Chain: A
Length: 539 amino acids
Theoretical weight: 60.88 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P31645 (Residues: 79-617; Coverage: 86%)
Gene names: HTT, SERT, SLC6A4
Sequence domains: Sodium:neurotransmitter symporter family
variable domain of 15B8 antibody Fab heavy chain Chain: B
Molecule details ›
Chain: B
Length: 118 amino acids
Theoretical weight: 12.98 KDa
Source organism: Mus musculus
variable domain of 15B8 antibody Fab light chain Chain: C
Molecule details ›
Chain: C
Length: 110 amino acids
Theoretical weight: 11.78 KDa
Source organism: Mus musculus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Ligand NAG 2 x NAG
5 bound ligands:
Ligand R16 1 x R16
Ligand D10 1 x D10
Ligand D12 3 x D12
Ligand HP6 6 x HP6
Ligand LNK 1 x LNK
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-23363
Expression system: Homo sapiens

Quick links

Citations

3 review citations

Applications and Potential of In Silico Approaches for Psychedelic Chemistry.
Karabulut et al. (2023)
2 more

1 mention without citation

Illumination of serotonin transporter mechanism and role of the allosteric site.
Yang et al. (2021)