› 7lez

Electron Microscopy
4.15Å resolution

Function and Biology Details

Reaction catalysed: 
L-arginine + H(2)O = L-ornithine + urea
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209178 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
mAb1 heavy chain Chains: D, G, Q, R
Molecule details ›
Chains: D, G, Q, R
Length: 455 amino acids
Theoretical weight: 50.27 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
mAb1 light chain Chains: E, I, O, P
Molecule details ›
Chains: E, I, O, P
Length: 214 amino acids
Theoretical weight: 23.49 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Arginase-1 Chains: A, B, C, J, M, N
Molecule details ›
Chains: A, B, C, J, M, N
Length: 322 amino acids
Theoretical weight: 34.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P05089 (Residues: 1-322; Coverage: 100%)
Gene name: ARG1
Sequence domains: Arginase family

Ligands and Environments

1 bound ligand:
Ligand MN 12 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.15Å
Relevant EMDB volumes: EMD-23295
Expression systems:
  • Cricetulus griseus
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 mention without citation

Cryo-EM structures of inhibitory antibodies complexed with arginase 1 provide insight into mechanism of action.
Palte et al. (2021)