7lbg

Electron Microscopy
2.6Å resolution

CryoEM structure of the HCMV Trimer gHgLgO in complex with human Transforming growth factor beta receptor type 3 and neutralizing fabs 13H11 and MSL-109

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-207851 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (10 distinct):
Envelope glycoprotein H Chain: A
Molecule details ›
Chain: A
Length: 767 amino acids
Theoretical weight: 87.31 KDa
Source organism: Human herpesvirus 5 strain Merlin
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6SW67 (Residues: 1-715; Coverage: 96%)
Gene names: UL75, gH
Sequence domains:
Envelope glycoprotein L Chain: B
Molecule details ›
Chain: B
Length: 278 amino acids
Theoretical weight: 30.85 KDa
Source organism: Human herpesvirus 5 strain Merlin
Expression system: Homo sapiens
UniProt:
  • Canonical: F5HCH8 (Residues: 1-278; Coverage: 100%)
Gene names: UL115, gL
Sequence domains: Cytomegalovirus glycoprotein L
Envelope glycoprotein O Chain: C
Molecule details ›
Chain: C
Length: 504 amino acids
Theoretical weight: 58.3 KDa
Source organism: Human betaherpesvirus 5
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8BCU3 (Residues: 1-464; Coverage: 100%)
Gene name: UL74
Sequence domains: Herpes UL74 glycoproteins
Transforming growth factor beta receptor type 3 Chain: D
Molecule details ›
Chain: D
Length: 787 amino acids
Theoretical weight: 87.36 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q03167 (Residues: 1-781; Coverage: 92%)
Gene name: TGFBR3
Sequence domains: Zona pellucida-like domain
Fab 13H11 light chain Chain: E
Molecule details ›
Chain: E
Length: 237 amino acids
Theoretical weight: 25.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Fab 13H11 heavy chain Chain: F
Molecule details ›
Chain: F
Length: 250 amino acids
Theoretical weight: 26.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Fab MSL-109 light chain Chain: G
Molecule details ›
Chain: G
Length: 257 amino acids
Theoretical weight: 28.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Fab MSL-109 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 257 amino acids
Theoretical weight: 27.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.6Å
Relevant EMDB volumes: EMD-23254
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda
  • Escherichia coli