› 7l0h

X-ray diffraction
2.1Å resolution

Vanadate-bound PTP1B T177G

Released:
DOI: 10.2210/pdb7l0h/pdb
PDB entry 7l0h coloured by chain, front view.
PDB entry 7l0h coloured by chain, side view.
PDB entry 7l0h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Single Residue on the WPD-Loop Affects the pH Dependency of Catalysis in Protein Tyrosine Phosphatases.
Shen R, Crean RM, Johnson SJ, Kamerlin SCL, Hengge AC
JACS Au 1 646-659 (2021)
PMID: 34308419

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 37.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-321; Coverage: 74%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase

Ligands and Environments

1 bound ligand:
Ligand VO4 1 x VO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P3121
Unit cell:
a: 88.471Å b: 88.471Å c: 104.979Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.178 0.208
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Insights into the importance of WPD-loop sequence for activity and structure in protein tyrosine phosphatases.
Shen et al. (2022)
1 more

2 mentions without citation

Interaction of Vanadium Complexes with Proteins: Revisiting the Reported Structures in the Protein Data Bank (PDB) since 2015.
Santos et al. (2023)
1 more