› 7ko7

Electron Microscopy
8.3Å resolution

Structure of the native cardiac thin filament at pCa=5.8 having upper Tn in Ca2+ free state and lower Tn in Ca2+ bound state

Released:
DOI: 10.2210/pdb7ko7/pdb
PDB entry 7ko7 coloured by chain, front view.
PDB entry 7ko7 coloured by chain, side view.
PDB entry 7ko7 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
The structure of the native cardiac thin filament at systolic Ca2+ levels.
Risi CM, Pepper I, Belknap B, Landim-Vieira M, White HD, Dryden K, Pinto JR, Chase PB, Galkin VE
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33753506
Related structures: EMD-22966

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 29-mer (preferred)
PDBe Complex ID:
PDB-CPX-236792 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Actin, alpha skeletal muscle Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O
Length: 375 amino acids
Theoretical weight: 41.86 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P68137 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Tropomyosin alpha-1 chain Chains: P, Q, R, S, W, X, Y, Z
Molecule details ›
Chains: P, Q, R, S, W, X, Y, Z
Length: 286 amino acids
Theoretical weight: 32.92 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P42639 (Residues: 1-284; Coverage: 100%)
Gene name: TPM1
Sequence domains: Tropomyosin
Troponin T, cardiac muscle Chains: T, a
Molecule details ›
Chains: T, a
Length: 186 amino acids
Theoretical weight: 23.02 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1SIP4 (Residues: 81-184, 185-265; Coverage: 66%)
Gene name: TNNT2
Sequence domains: Troponin
Troponin I, cardiac muscle Chains: U, b
Molecule details ›
Chains: U, b
Length: 170 amino acids
Theoretical weight: 19.64 KDa
Source organism: Sus scrofa
Troponin C, slow skeletal and cardiac muscles Chains: V, c
Molecule details ›
Chains: V, c
Length: 160 amino acids
Theoretical weight: 18.29 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P63317 (Residues: 2-161; Coverage: 99%)
Gene names: TNNC, TNNC1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.3Å
Relevant EMDB volumes: EMD-22966

Quick links

Citations

9 review citations

Small Angle X-ray Diffraction as a Tool for Structural Characterization of Muscle Disease.
Ma et al. (2022)
8 more