7k7g

Electron Microscopy
4.2Å resolution

nucleosome and Gal4 complex

Released:
Related structures: EMD-22698

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-121443 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
Histone H3 Chains: A, E
Molecule details ›
Chains: A, E
Length: 136 amino acids
Theoretical weight: 15.39 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia phage EcSzw-2
UniProt:
  • Canonical: P61830 (Residues: 1-136; Coverage: 100%)
Gene names: HHT1, HHT2, N2749, SIN2, YBR010W, YBR0201, YNL031C
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 103 amino acids
Theoretical weight: 11.4 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia phage EcSzw-2
UniProt:
  • Canonical: P02309 (Residues: 1-103; Coverage: 100%)
Gene names: HHF1, HHF2, N2752, YBR009C, YBR0122, YNL030W
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A.1 Chains: C, G
Molecule details ›
Chains: C, G
Length: 132 amino acids
Theoretical weight: 14.01 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia phage EcSzw-2
UniProt:
  • Canonical: P04911 (Residues: 1-132; Coverage: 100%)
Gene names: H2A1, HTA1, SPT11, YD9934.10, YDR225W
Sequence domains:
Histone H2B.1 Chains: D, H
Molecule details ›
Chains: D, H
Length: 131 amino acids
Theoretical weight: 14.28 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia phage EcSzw-2
UniProt:
  • Canonical: P02293 (Residues: 1-131; Coverage: 100%)
Gene names: H2B1, HTB1, SPT12, YD9934.09C, YDR224C
Sequence domains: Core histone H2A/H2B/H3/H4
Centromere DNA-binding protein complex CBF3 subunit B Chain: M
Molecule details ›
Chain: M
Length: 48 amino acids
Theoretical weight: 5.61 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40969 (Residues: 1-48; Coverage: 8%)
Gene names: CBF3, CBF3B, CEP3, CSL1, YM8520.17C, YMR168C
Sequence domains: Fungal Zn(2)-Cys(6) binuclear cluster domain
DNA (147-MER) Chain: I
Molecule details ›
Chain: I
Length: 147 nucleotides
Theoretical weight: 45.33 KDa
DNA (147-MER) Chain: J
Molecule details ›
Chain: J
Length: 147 nucleotides
Theoretical weight: 45.4 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-22698
Expression systems:
  • Escherichia phage EcSzw-2
  • Saccharomyces cerevisiae