7jk4

Electron Microscopy
3.4Å resolution

Structure of Drosophila ORC bound to AT-rich DNA and Cdc6

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-121344 (preferred)
Entry contents:
7 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (9 distinct):
Origin recognition complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 618 amino acids
Theoretical weight: 69.09 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q24168 (Residues: 1-618; Coverage: 100%)
Gene names: CG3041, Orc2
Sequence domains: Origin recognition complex subunit 2
Origin recognition complex subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 721 amino acids
Theoretical weight: 82.36 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q7K2L1 (Residues: 1-721; Coverage: 100%)
Gene names: CG34315-RB, CG4088, DmORC3, DmOrc3, Dmel\CG4088, Dmel_CG4088, LAT, Lat, ORC, ORC3, Orc3, l(2)49Fd, l(2)vr6, lat, lat-RA, latheo, orc3, vr-6, vr6
Sequence domains:
Origin recognition complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 462 amino acids
Theoretical weight: 52.28 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9W102 (Residues: 1-459; Coverage: 100%)
Gene names: CG2917, DmORC4, Dmel\CG2917, Dmel_CG2917, ORC, ORC4, Orc4, dmOrc4, orc4, rDmORC
Sequence domains:
Origin recognition complex subunit 5 Chain: E
Molecule details ›
Chain: E
Length: 460 amino acids
Theoretical weight: 52.18 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q24169 (Residues: 1-460; Coverage: 100%)
Gene names: CG7833, Orc5
Sequence domains:
Origin recognition complex subunit 6 Chain: F
Molecule details ›
Chain: F
Length: 257 amino acids
Theoretical weight: 29.28 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9Y1B2 (Residues: 1-257; Coverage: 100%)
Gene names: CG1584, Orc6
Sequence domains: Origin recognition complex subunit 6 (ORC6)
Origin recognition complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 488 amino acids
Theoretical weight: 54.49 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O16810 (Residues: 440-924; Coverage: 53%)
Gene names: CG10667, Orc1
Sequence domains:
Cell division control protein Chain: G
Molecule details ›
Chain: G
Length: 424 amino acids
Theoretical weight: 48.05 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9VSM9 (Residues: 242-662; Coverage: 64%)
Gene names: CDC6, CG5971, Cdc6, DmCdc6, Dmel\CG5971, Dmel_CG5971, cdc6
Sequence domains:
DNA (34-MER) Chain: H
Molecule details ›
Chain: H
Length: 60 nucleotides
Theoretical weight: 18.48 KDa
DNA (34-MER) Chain: I
Molecule details ›
Chain: I
Length: 60 nucleotides
Theoretical weight: 18.49 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-22361
Expression system: Trichoplusia ni