› 7eyx

X-ray diffraction
1.82Å resolution

Crystal structure of Mg-free KRAS-G12D bound to GDP

Released:
DOI: 10.2210/pdb7eyx/pdb
PDB entry 7eyx coloured by chain, front view.
PDB entry 7eyx coloured by chain, side view.
PDB entry 7eyx coloured by chain, top view.
Source organism: Homo sapiens
Entry author: Fei Z

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase KRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 2-169; Coverage: 89%)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 36.01Å b: 37.34Å c: 100.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.212
Expression system: Escherichia coli

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