› 7egi

Electron Microscopy
9.82Å resolution

TFIID in rearranged conformation

Released:
DOI: 10.2210/pdb7egi/pdb
PDB entry 7egi coloured by chain, front view.
PDB entry 7egi coloured by chain, side view.
PDB entry 7egi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into preinitiation complex assembly on core promoters.
Chen X, Qi Y, Wu Z, Wang X, Li J, Zhao D, Hou H, Li Y, Yu Z, Liu W, Wang M, Ren Y, Li Z, Yang H, Xu Y
Science 372 (2021)
PMID: 33795473
Related structures: EMD-31118

Function and Biology Details

Reactions catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 22-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126221 (preferred)
Entry contents:
16 distinct polypeptide molecules
Macromolecules (16 distinct):
Transcription initiation factor TFIID subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 1872 amino acids
Theoretical weight: 212.96 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P21675 (Residues: 1-1893; Coverage: 99%)
Gene names: BA2R, CCG1, CCGS, TAF1, TAF2A
Sequence domains:
Transcription initiation factor TFIID subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 1199 amino acids
Theoretical weight: 137.16 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6P1X5 (Residues: 1-1199; Coverage: 100%)
Gene names: CIF150, TAF2, TAF2B
Transcription initiation factor TFIID subunit 4 Chains: D, d
Molecule details ›
Chains: D, d
Length: 1085 amino acids
Theoretical weight: 110.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O00268 (Residues: 1-1085; Coverage: 100%)
Gene names: TAF2C, TAF2C1, TAF4, TAF4A, TAFII130, TAFII135
Sequence domains:
Transcription initiation factor TFIID subunit 5 Chains: E, e
Molecule details ›
Chains: E, e
Length: 800 amino acids
Theoretical weight: 86.93 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15542 (Residues: 1-800; Coverage: 100%)
Gene names: TAF2D, TAF5
Sequence domains:
Transcription initiation factor TFIID subunit 6 Chains: F, f
Molecule details ›
Chains: F, f
Length: 677 amino acids
Theoretical weight: 72.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P49848 (Residues: 1-677; Coverage: 100%)
Gene names: TAF2E, TAF6, TAFII70
Sequence domains:
Transcription initiation factor TFIID subunit 7 Chain: G
Molecule details ›
Chain: G
Length: 349 amino acids
Theoretical weight: 40.33 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15545 (Residues: 1-349; Coverage: 100%)
Gene names: TAF2F, TAF7, TAFII55
Sequence domains: TAFII55 protein conserved region
Transcription initiation factor TFIID subunit 8 Chain: H
Molecule details ›
Chain: H
Length: 310 amino acids
Theoretical weight: 34.3 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q7Z7C8 (Residues: 1-310; Coverage: 100%)
Gene names: TAF8, TAFII43, TBN
Sequence domains:
Transcription initiation factor TFIID subunit 9 Chains: I, i
Molecule details ›
Chains: I, i
Length: 264 amino acids
Theoretical weight: 29.01 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q16594 (Residues: 1-264; Coverage: 100%)
Gene names: TAF2G, TAF9, TAFII31
Sequence domains: Transcription initiation factor IID, 31kD subunit
Transcription initiation factor TFIID subunit 10 Chains: J, j
Molecule details ›
Chains: J, j
Length: 218 amino acids
Theoretical weight: 21.73 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q12962 (Residues: 1-218; Coverage: 100%)
Gene names: TAF10, TAF2A, TAF2H, TAFII30
Sequence domains: Transcription initiation factor TFIID 23-30kDa subunit
Transcription initiation factor TFIID subunit 12 Chains: L, l
Molecule details ›
Chains: L, l
Length: 161 amino acids
Theoretical weight: 17.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q16514 (Residues: 1-161; Coverage: 100%)
Gene names: TAF12, TAF15, TAF2J, TAFII20
Sequence domains: Transcription initiation factor TFIID subunit A
Transcription initiation factor IIA subunit 2 Chain: O
Molecule details ›
Chain: O
Length: 109 amino acids
Theoretical weight: 12.47 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P52657 (Residues: 1-109; Coverage: 100%)
Gene names: GTF2A2, TF2A2
Sequence domains:
TATA-box-binding protein Chain: P
Molecule details ›
Chain: P
Length: 339 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P20226 (Residues: 1-339; Coverage: 100%)
Gene names: GTF2D1, TBP, TF2D, TFIID
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Transcription initiation factor IIA subunit 1 Chain: Q
Molecule details ›
Chain: Q
Length: 376 amino acids
Theoretical weight: 41.54 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P52655 (Residues: 1-376; Coverage: 100%)
Gene names: GTF2A1, TF2A1
Sequence domains: Transcription factor IIA, alpha/beta subunit
Transcription initiation factor TFIID subunit 3 Chain: c
Molecule details ›
Chain: c
Length: 929 amino acids
Theoretical weight: 103.77 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5VWG9 (Residues: 1-929; Coverage: 100%)
Gene name: TAF3
Sequence domains:
Transcription initiation factor TFIID subunit 11 Chain: k
Molecule details ›
Chain: k
Length: 211 amino acids
Theoretical weight: 23.34 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15544 (Residues: 1-211; Coverage: 100%)
Gene names: PRO2134, TAF11, TAF2I
Sequence domains: hTAFII28-like protein conserved region
Transcription initiation factor TFIID subunit 13 Chain: m
Molecule details ›
Chain: m
Length: 124 amino acids
Theoretical weight: 14.31 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15543 (Residues: 1-124; Coverage: 100%)
Gene names: TAF13, TAF2K, TAFII18
Sequence domains: Transcription initiation factor IID, 18kD subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.82Å
Relevant EMDB volumes: EMD-31118
Expression system: Homo sapiens

Quick links

Citations

13 review citations

The Mediator complex as a master regulator of transcription by RNA polymerase II.
Richter et al. (2022)
12 more