7e4q

X-ray diffraction
2.5Å resolution

Crystal structure of tubulin in complex with L-DM1-SMe

Released:
Primary publication:
C3 ester side chain plays a pivotal role in the antitumor activity of Maytansinoids.
Biochem Biophys Res Commun 566 197-203 (2021)
PMID: 34144258

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-237179 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 440 amino acids
Theoretical weight: 48.97 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P81947 (Residues: 1-440; Coverage: 98%)
Sequence domains:
Tubulin beta-2B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 431 amino acids
Theoretical weight: 48.39 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6B856 (Residues: 1-431; Coverage: 97%)
Gene name: TUBB2B
Sequence domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 138 amino acids
Theoretical weight: 16.28 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63043 (Residues: 50-187; Coverage: 73%)
Gene name: Stmn4
Sequence domains: Stathmin family
Tubulin tyrosine ligase Chain: F
Molecule details ›
Chain: F
Length: 380 amino acids
Theoretical weight: 43.83 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 104.893Å b: 157.209Å c: 181.658Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.205 0.252
Expression system: Escherichia coli