› 7czf

X-ray diffraction
3.2Å resolution

Crystal structure of Kaposi Sarcoma associated herpesvirus (KSHV ) gHgL in complex with the ligand binding domian (LBD) of EphA2

Released:
DOI: 10.2210/pdb7czf/pdb
PDB entry 7czf coloured by chain, front view.
PDB entry 7czf coloured by chain, side view.
PDB entry 7czf coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis of EphA2 recognition by gHgL from gammaherpesviruses.
Su C, Wu L, Chai Y, Qi J, Tan S, Gao GF, Song H, Yan J
Nat Commun 11 5964 (2020)
PMID: 33235207

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-151396 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (6 distinct):
Ephrin type-A receptor 2 Chains: A, D
Molecule details ›
Chains: A, D
Length: 185 amino acids
Theoretical weight: 21.32 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P29317 (Residues: 28-206; Coverage: 19%)
Gene names: ECK, EPHA2
Sequence domains: Ephrin receptor ligand binding domain
Herpesvirus glycoprotein H C-terminal domain-containing protein Chains: B, E
Molecule details ›
Chains: B, E
Length: 677 amino acids
Theoretical weight: 75.37 KDa
Source organism: Human gammaherpesvirus 8
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q98142 (Residues: 22-698; Coverage: 93%)
Gene names: HHV8GK18_gp26, ORF22
Sequence domains:
ORF47 Chains: C, F
Molecule details ›
Chains: C, F
Length: 157 amino acids
Theoretical weight: 17.09 KDa
Source organism: Human gammaherpesvirus 8
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q76RG7 (Residues: 21-167; Coverage: 88%)
Gene names: HHV8GK18_gp49, ORF46, ORF47
Sequence domains: Viral glycoprotein L

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
1 bound ligand:
Ligand NAG 12 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 80.803Å b: 153.196Å c: 275.228Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.239 0.272
Expression system: Trichoplusia ni

Quick links

Citations

4 review citations

Cellular Receptors Involved in KSHV Infection.
van der Meulen et al. (2021)
3 more

2 mentions without citation

Well Put Together-A Guide to Accessorizing with the Herpesvirus gH/gL Complexes.
Gonzalez-Del Pino et al. (2022)
1 more