› 7cob

X-ray diffraction
1.8Å resolution

Ternary complex of DNA polymerase Mu (Q441A) with 1-nt gapped DNA (T:dGMPNPP)

Released:
DOI: 10.2210/pdb7cob/pdb
PDB entry 7cob coloured by chain, front view.
PDB entry 7cob coloured by chain, side view.
PDB entry 7cob coloured by chain, top view.
Source organisms:
Primary publication:
Mechanism of genome instability mediated by human DNA polymerase mu misincorporation.
Guo M, Wang Y, Tang Y, Chen Z, Hou J, Dai J, Wang Y, Wang L, Xu H, Tian B, Hua Y, Zhao Y
Nat Commun 12 3759 (2021)
PMID: 34145298

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121161 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA-directed DNA/RNA polymerase mu Chain: A
Molecule details ›
Chain: A
Length: 482 amino acids
Theoretical weight: 53.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NP87 (Residues: 1-494; Coverage: 98%)
Gene names: POLM, polmu
Sequence domains:
DNA (5'-D(*CP*GP*GP*CP*TP*TP*AP*CP*G)-3') Chain: T
Molecule details ›
Chain: T
Length: 9 nucleotides
Theoretical weight: 2.73 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*GP*TP*A)-3') Chain: P
Molecule details ›
Chain: P
Length: 4 nucleotides
Theoretical weight: 1.19 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*GP*CP*CP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 4 nucleotides
Theoretical weight: 1.19 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand XG4 1 x XG4
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 59.76Å b: 68.34Å c: 110.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.204
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

For the Better or for the Worse? The Effect of Manganese on the Activity of Eukaryotic DNA Polymerases.
Balint et al. (2023)
2 other articles

1 mention without citation

Mechanism of genome instability mediated by human DNA polymerase mu misincorporation.
Guo et al. (2021)