› 7bwn

X-ray diffraction
2.4Å resolution

Crystal Structure of a Designed Protein Heterocatenane

Released:
DOI: 10.2210/pdb7bwn/pdb
PDB entry 7bwn coloured by chain, front view.
PDB entry 7bwn coloured by chain, side view.
PDB entry 7bwn coloured by chain, top view.
Source organisms:
Primary publication:
Cellular Synthesis and X-ray Crystal Structure of a Designed Protein Heterocatenane.
Liu Y, Duan Z, Fang J, Zhang F, Xiao J, Zhang WB
Angew Chem Int Ed Engl 59 16122-16127 (2020)
PMID: 32506656

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138102 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cellular tumor antigen p53; Green fluorescent protein Chains: A, C, E, F, H, J, M, O
Molecule details ›
Chains: A, C, E, F, H, J, M, O
Length: 280 amino acids
Theoretical weight: 31.62 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
  • Canonical: P04637 (Residues: 322-356; Coverage: 9%)
Gene names: GFP, P53, TP53
Sequence domains:
Cellular tumor antigen p53 Chains: B, D, G, I, K, L, N, P
Molecule details ›
Chains: B, D, G, I, K, L, N, P
Length: 46 amino acids
Theoretical weight: 5.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 326-356; Coverage: 8%)
Gene names: P53, TP53
Sequence domains: P53 tetramerisation motif

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R3
Unit cell:
a: 108.897Å b: 108.897Å c: 684.966Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.187 0.239
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Genetically engineered materials: Proteins and beyond.
Wei et al. (2022)
1 more

4 mentions without citation

Immunoregulatory mechanism studies of ginseng leaves on lung cancer based on network pharmacology and molecular docking.
Li et al. (2021)
3 more