› 7bt2

X-ray diffraction
3Å resolution

Crystal structure of the SERCA2a in the E2.ATP state

Released:
DOI: 10.2210/pdb7bt2/pdb
PDB entry 7bt2 coloured by chain, front view.
PDB entry 7bt2 coloured by chain, side view.
PDB entry 7bt2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
What ATP binding does to the Ca2+ pump and how nonproductive phosphoryl transfer is prevented in the absence of Ca2.
Kabashima Y, Ogawa H, Nakajima R, Toyoshima C
Proc Natl Acad Sci U S A 117 18448-18458 (2020)
PMID: 32675243

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147779 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 Chain: A
Molecule details ›
Chain: A
Length: 997 amino acids
Theoretical weight: 109.8 KDa
Source organism: Homo sapiens
Expression system: Chlorocebus sabaeus
UniProt:
  • Canonical: P16615 (Residues: 1-993; Coverage: 95%)
Gene names: ATP2A2, ATP2B
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ATP 1 x ATP
Ligand K 1 x K
Ligand PCW 4 x PCW
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2221
Unit cell:
a: 121.604Å b: 270.382Å c: 97.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.222
Expression system: Chlorocebus sabaeus

Quick links

Citations

2 review citations

P-type ATPases: Many more enigmas left to solve.
Palmgren M. (2023)
1 more

1 mention without citation

What ATP binding does to the Ca2+ pump and how nonproductive phosphoryl transfer is prevented in the absence of Ca2.
Kabashima et al. (2020)