› 7bmr

X-ray diffraction
1.78Å resolution

HEWL in cesium chloride (0.25 M CsCl in protein buffer and 1.71 M CsCl in cryo protectant)

Released:
DOI: 10.2210/pdb7bmr/pdb
PDB entry 7bmr coloured by chain, front view.
PDB entry 7bmr coloured by chain, side view.
PDB entry 7bmr coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Cesium based phasing of macromolecules: a general easy to use approach for solving the phase problem.
Koelmel W, Kuper J, Kisker C
Sci Rep 11 17038 (2021)
PMID: 34426585

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family

Ligands and Environments

3 bound ligands:
Ligand CL 3 x CL
Ligand EDO 2 x EDO
Ligand CS 16 x CS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P43212
Unit cell:
a: 78.968Å b: 78.968Å c: 36.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.212

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Citations

1 review citation

SARS-CoV-2 proteins structural studies using synchrotron radiation.
Kosenko et al. (2023)