› 7ast

Electron Microscopy
4Å resolution

Apo Human RNA Polymerase III

Released:
DOI: 10.2210/pdb7ast/pdb
PDB entry 7ast coloured by chain, front view.
PDB entry 7ast coloured by chain, side view.
PDB entry 7ast coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human RNA polymerase III.
Ramsay EP, Abascal-Palacios G, Daiß JL, King H, Gouge J, Pilsl M, Beuron F, Morris E, Gunkel P, Engel C, Vannini A
Nat Commun 11 6409 (2020)
PMID: 33335104
Related structures: EMD-11904

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptadecamer (preferred)
PDBe Complex ID:
PDB-CPX-127154 (preferred)
Entry contents:
17 distinct polypeptide molecules
Macromolecules (17 distinct):
DNA-directed RNA polymerase III subunit RPC1 Chain: N
Molecule details ›
Chain: N
Length: 1390 amino acids
Theoretical weight: 155.86 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O14802 (Residues: 1-1390; Coverage: 100%)
Gene name: POLR3A
Sequence domains:
DNA-directed RNA polymerase III subunit RPC10 Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 12.35 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y2Y1 (Residues: 1-108; Coverage: 100%)
Gene names: My010, POLR3K, RPC11
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: B
Molecule details ›
Chain: B
Length: 67 amino acids
Theoretical weight: 7.66 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62875 (Residues: 1-67; Coverage: 100%)
Gene name: POLR2L
Sequence domains: RNA polymerases N / 8 kDa subunit
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chain: C
Molecule details ›
Chain: C
Length: 58 amino acids
Theoretical weight: 7.02 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P53803 (Residues: 1-58; Coverage: 100%)
Gene name: POLR2K
Sequence domains: DNA directed RNA polymerase, 7 kDa subunit
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: D
Molecule details ›
Chain: D
Length: 150 amino acids
Theoretical weight: 17.16 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P52434 (Residues: 1-150; Coverage: 100%)
Gene name: POLR2H
Sequence domains: RNA polymerase Rpb8
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: E
Molecule details ›
Chain: E
Length: 127 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61218 (Residues: 1-127; Coverage: 100%)
Gene names: POLR2F, POLRF
Sequence domains: RNA polymerase Rpb6
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: F
Molecule details ›
Chain: F
Length: 210 amino acids
Theoretical weight: 24.58 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P19388 (Residues: 1-210; Coverage: 100%)
Gene name: POLR2E
Sequence domains:
DNA-directed RNA polymerases I and III subunit RPAC2 Chain: G
Molecule details ›
Chain: G
Length: 133 amino acids
Theoretical weight: 15.26 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0DPB6 (Residues: 1-133; Coverage: 100%)
Gene name: POLR1D
Sequence domains:
DNA-directed RNA polymerases I and III subunit RPAC1 Chain: H
Molecule details ›
Chain: H
Length: 346 amino acids
Theoretical weight: 39.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O15160 (Residues: 1-346; Coverage: 100%)
Gene names: POLR1C, POLR1E
Sequence domains:
DNA-directed RNA polymerase III subunit RPC9 Chain: I
Molecule details ›
Chain: I
Length: 148 amino acids
Theoretical weight: 16.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O75575 (Residues: 1-148; Coverage: 100%)
Gene name: CRCP
Sequence domains: RNA polymerase Rpb4
DNA-directed RNA polymerase III subunit RPC8 Chain: J
Molecule details ›
Chain: J
Length: 204 amino acids
Theoretical weight: 22.94 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y535 (Residues: 1-204; Coverage: 100%)
Gene names: KIAA1665, POLR3H, RPC8
Sequence domains:
DNA-directed RNA polymerase III subunit RPC5 Chain: K
Molecule details ›
Chain: K
Length: 708 amino acids
Theoretical weight: 80 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9NVU0 (Residues: 1-708; Coverage: 100%)
Gene names: KIAA1452, POLR3E
Sequence domains:
DNA-directed RNA polymerase III subunit RPC4 Chain: L
Molecule details ›
Chain: L
Length: 398 amino acids
Theoretical weight: 44.47 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P05423 (Residues: 1-398; Coverage: 100%)
Gene names: BN51, BN51T, POLR3D
Sequence domains: RNA polymerase III RPC4
DNA-directed RNA polymerase III subunit RPC2 Chain: M
Molecule details ›
Chain: M
Length: 1133 amino acids
Theoretical weight: 127.95 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9NW08 (Residues: 1-1133; Coverage: 100%)
Gene name: POLR3B
Sequence domains:
DNA-directed RNA polymerase III subunit RPC6 Chain: Z
Molecule details ›
Chain: Z
Length: 316 amino acids
Theoretical weight: 35.73 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9H1D9 (Residues: 1-316; Coverage: 100%)
Gene name: POLR3F
Sequence domains: RNA polymerase Rpc34 subunit
DNA-directed RNA polymerase III subunit RPC3 Chain: X
Molecule details ›
Chain: X
Length: 534 amino acids
Theoretical weight: 60.69 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BUI4 (Residues: 1-534; Coverage: 100%)
Gene name: POLR3C
Sequence domains:
DNA-directed RNA polymerase III subunit RPC7-beta Chain: Y
Molecule details ›
Chain: Y
Length: 36 amino acids
Theoretical weight: 3.08 KDa
Source organism: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-11904

Quick links

Citations

12 review citations

Battle Royale: Innate Recognition of Poxviruses and Viral Immune Evasion.
Yu et al. (2021)
11 more

1 mention without citation

The human RNA polymerase I structure reveals an HMG-like docking domain specific to metazoans.
Daiß et al. (2022)