› 7apl

X-ray diffraction
1.99Å resolution

tRNA-guanine transglycosylase G87C mutant spin-labeled with MTSL

Released:
DOI: 10.2210/pdb7apl/pdb
PDB entry 7apl coloured by chain, front view.
PDB entry 7apl coloured by chain, side view.
PDB entry 7apl coloured by chain, top view.
Source organism: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Primary publication:
Unraveling a Ligand-Induced Twist of a Homodimeric Enzyme by Pulsed Electron-Electron Double Resonance.
Nguyen D, Abdullin D, Heubach CA, Pfaffeneder T, Nguyen A, Heine A, Reuter K, Diederich F, Schiemann O, Klebe G
Angew Chem Int Ed Engl 60 23419-23426 (2021)
PMID: 34387025

Function and Biology Details

Reaction catalysed: 
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Queuine tRNA-ribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.27 KDa
Source organism: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28720 (Residues: 1-386; Coverage: 100%)
Gene names: ZMO0363, tgt
Sequence domains: Queuine tRNA-ribosyltransferase

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand GOL 2 x GOL
1 modified residue:
Ligand R1A 1 x R1A

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2221
Unit cell:
a: 64.542Å b: 90.947Å c: 167.818Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Unraveling a Ligand-Induced Twist of a Homodimeric Enzyme by Pulsed Electron-Electron Double Resonance.
Nguyen et al. (2021)