› 7ad7

X-ray diffraction
2.3Å resolution

Crystal structure of human complement C5 in complex with the K8 bovine knob domain peptide.

Released:
DOI: 10.2210/pdb7ad7/pdb
PDB entry 7ad7 coloured by chain, front view.
PDB entry 7ad7 coloured by chain, side view.
PDB entry 7ad7 coloured by chain, top view.
Source organisms:
Primary publication:
The allosteric modulation of complement C5 by knob domain peptides.
Macpherson A, Laabei M, Ahdash Z, Graewert MA, Birtley JR, Schulze ME, Crennell S, Robinson SA, Holmes B, Oleinikovas V, Nilsson PH, Snowden J, Ellis V, Mollnes TE, Deane CM, Svergun D, Lawson AD, van den Elsen JM
Elife 10 (2021)
PMID: 33570492

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133880 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Complement C5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1676 amino acids
Theoretical weight: 188.51 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01031 (Residues: 1-1676; Coverage: 100%)
Gene names: C5, CPAMD4
Sequence domains:
K8 peptide Chain: C
Molecule details ›
Chain: C
Length: 52 amino acids
Theoretical weight: 5.96 KDa
Source organism: Bos taurus
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand EOH 4 x EOH
Ligand MHA 1 x MHA
Ligand MPD 2 x MPD
Ligand CYS 1 x CYS
Ligand TAM 1 x TAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 81.559Å b: 161.695Å c: 187.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.234
Expression system: Homo sapiens

Quick links

Citations

10 citation in other articles

Milking the Cow: Cattle-Derived Chimeric Ultralong CDR-H3 Antibodies and Their Engineered CDR-H3-Only Knobbody Counterparts Targeting Epidermal Growth Factor Receptor Elicit Potent NK Cell-Mediated Cytotoxicity.
Pekar et al. (2021)
9 more

2 mentions without citation

The allosteric modulation of complement C5 by knob domain peptides.
Macpherson et al. (2021)
1 more