› 7a3x

X-ray diffraction
1.85Å resolution

tRNA-guanine transglycosylase C158S/C281S/Y330C/H333A mutant in complex with sulfolane

Released:
DOI: 10.2210/pdb7a3x/pdb
PDB entry 7a3x coloured by chain, front view.
PDB entry 7a3x coloured by chain, side view.
PDB entry 7a3x coloured by chain, top view.
Source organism: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Primary publication:
Targeting a Cryptic Pocket in a Protein-Protein Contact by Disulfide-Induced Rupture of a Homodimeric Interface.
Nguyen D, Xie X, Jakobi S, Terwesten F, Metz A, Nguyen TXP, Palchykov VA, Heine A, Reuter K, Klebe G
ACS Chem Biol 16 1090-1098 (2021)
PMID: 34081441

Function and Biology Details

Reaction catalysed: 
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Queuine tRNA-ribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 42.91 KDa
Source organism: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28720 (Residues: 1-386; Coverage: 100%)
Gene names: ZMO0363, tgt
Sequence domains: Queuine tRNA-ribosyltransferase

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand GOL 2 x GOL
Ligand QXB 3 x QXB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6522
Unit cell:
a: 128.292Å b: 128.292Å c: 114.859Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.203 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

LifeSoaks: a tool for analyzing solvent channels in protein crystals and obstacles for soaking experiments.
Pletzer-Zelgert et al. (2023)