› 6zk6

X-ray diffraction
1.9Å resolution

Protein Phosphatase 1 (PP1) T320E mutant

Released:
DOI: 10.2210/pdb6zk6/pdb
PDB entry 6zk6 coloured by chain, front view.
PDB entry 6zk6 coloured by chain, side view.
PDB entry 6zk6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Towards Dissecting the Mechanism of Protein Phosphatase-1 Inhibition by Its C-Terminal Phosphorylation.
Salvi F, Hoermann B, Del Pino García J, Fontanillo M, Derua R, Beullens M, Bollen M, Barabas O, Köhn M
Chembiochem 22 834-838 (2021)
PMID: 33085143

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 37.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62136 (Residues: 1-330; Coverage: 100%)
Gene names: PPP1A, PPP1CA
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand FE 2 x FE
Ligand PO4 1 x PO4
2 modified residues:
Ligand CSD 1 x CSD
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Unit cell:
a: 38.546Å b: 68.749Å c: 127.846Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.194
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Regulation of Synaptic Transmission and Plasticity by Protein Phosphatase 1.
Foley et al. (2021)
2 more

1 mention without citation

Structure of the MRAS-SHOC2-PP1C phosphatase complex.
Hauseman et al. (2022)