› 6zga

Electron Microscopy
4.6Å resolution

COPII on membranes, inner coat

Released:
DOI: 10.2210/pdb6zga/pdb
PDB entry 6zga coloured by chain, front view.
PDB entry 6zga coloured by chain, side view.
PDB entry 6zga coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network.
Hutchings J, Stancheva VG, Brown NR, Cheung ACM, Miller EA, Zanetti G
Nat Commun 12 2034 (2021)
PMID: 33795673
Related structures: EMD-11199

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-147304 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Protein transport protein SEC23 Chains: A, E
Molecule details ›
Chains: A, E
Length: 767 amino acids
Theoretical weight: 85.33 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P15303 (Residues: 2-768; Coverage: 100%)
Gene names: P9705.14, SEC23, YPR181C
Sequence domains:
Protein transport protein SEC24 Chains: B, F
Molecule details ›
Chains: B, F
Length: 926 amino acids
Theoretical weight: 103.73 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P40482 (Residues: 1-926; Coverage: 100%)
Gene names: ANU1, SEC24, YIL109C
Sequence domains:
Small COPII coat GTPase SAR1 Chain: C
Molecule details ›
Chain: C
Length: 190 amino acids
Theoretical weight: 21.47 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P20606 (Residues: 1-190; Coverage: 100%)
Gene names: SAR1, YPL218W
Sequence domains: ADP-ribosylation factor family
PRO-PRO-PRO Chains: D, H
Molecule details ›
Chains: D, H
Length: 3 amino acids
Theoretical weight: 309 Da
Source organism: Saccharomyces cerevisiae
Expression system: Spodoptera frugiperda
Small COPII coat GTPase SAR1 Chain: G
Molecule details ›
Chain: G
Length: 189 amino acids
Theoretical weight: 21.36 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P20606 (Residues: 1-189; Coverage: 100%)
Gene names: SAR1, YPL218W
Sequence domains: ADP-ribosylation factor family

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand GNP 2 x GNP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.6Å
Relevant EMDB volumes: EMD-11199
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

7 review citations

Current data processing strategies for cryo-electron tomography and subtomogram averaging.
Pyle et al. (2021)
6 more