6z9t

Electron Microscopy
4.1Å resolution

Transcription termination intermediate complex 5

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-121011 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (9 distinct):
Transcription termination factor Rho Chains: a, b, c, d, e, f
Molecule details ›
Chains: a, b, c, d, e, f
Length: 419 amino acids
Theoretical weight: 47.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0AG30 (Residues: 1-419; Coverage: 100%)
Gene names: JW3756, b3783, nitA, psuA, rho, rnsC, sbaA, tsu
Sequence domains:
Transcription termination/antitermination protein NusA Chain: A
Molecule details ›
Chain: A
Length: 497 amino acids
Theoretical weight: 55.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0AFF6 (Residues: 1-495; Coverage: 100%)
Gene names: JW3138, b3169, nusA
Sequence domains:
DNA-directed RNA polymerase subunit alpha Chains: U, V
Molecule details ›
Chains: U, V
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-329; Coverage: 100%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: W
Molecule details ›
Chain: W
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Gene names: JW3624, b3649, rpoZ
Sequence domains: RNA polymerase Rpb6
DNA-directed RNA polymerase subunit beta Chain: X
Molecule details ›
Chain: X
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: Y
Molecule details ›
Chain: Y
Length: 1416 amino acids
Theoretical weight: 156.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A8T7 (Residues: 1-1407; Coverage: 100%)
Gene names: JW3951, b3988, rpoC, tabB
Sequence domains:
template strand Chain: L
Molecule details ›
Chain: L
Length: 50 nucleotides
Theoretical weight: 15.21 KDa
non template strand Chain: K
Molecule details ›
Chain: K
Length: 50 nucleotides
Theoretical weight: 15.54 KDa
rut RNA Chain: R
Molecule details ›
Chain: R
Length: 99 nucleotides
Theoretical weight: 31.69 KDa

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-11091
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'