› 6z88

X-ray diffraction
2.69Å resolution

human GTP cyclohydrolase I in complex with allosteric inhibitor

Released:
DOI: 10.2210/pdb6z88/pdb
PDB entry 6z88 coloured by chain, front view.
PDB entry 6z88 coloured by chain, side view.
PDB entry 6z88 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A hybrid approach reveals the allosteric regulation of GTP cyclohydrolase I.
Ebenhoch R, Prinz S, Kaltwasser S, Mills DJ, Meinecke R, Rübbelke M, Reinert D, Bauer M, Weixler L, Zeeb M, Vonck J, Nar H
Proc Natl Acad Sci U S A 117 31838-31849 (2020)
PMID: 33229582

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151801 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP cyclohydrolase 1 Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 224 amino acids
Theoretical weight: 25.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30793 (Residues: 41-250; Coverage: 84%)
Gene names: DYT5, GCH, GCH1
Sequence domains: GTP cyclohydrolase I

Ligands and Environments

2 bound ligands:
Ligand QBK 10 x QBK
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 112.355Å b: 161.505Å c: 271.642Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Tetrahydrobiopterin in Cell Function and Death Mechanisms.
Vasquez-Vivar et al. (2022)
3 other articles

1 mention without citation

A hybrid approach reveals the allosteric regulation of GTP cyclohydrolase I.
Ebenhoch et al. (2020)