› 6yu4

X-ray diffraction
2.26Å resolution

Crystal structure of MhsT in complex with L-4F-phenylalanine

Released:
DOI: 10.2210/pdb6yu4/pdb
PDB entry 6yu4 coloured by chain, front view.
PDB entry 6yu4 coloured by chain, side view.
PDB entry 6yu4 coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans
Primary publication:
A non-helical region in transmembrane helix 6 of hydrophobic amino acid transporter MhsT mediates substrate recognition.
Focht D, Neumann C, Lyons J, Eguskiza Bilbao A, Blunck R, Malinauskaite L, Schwarz IO, Javitch JA, Quick M, Nissen P
EMBO J 40 e105164 (2021)
PMID: 33155685

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sodium-dependent transporter Chain: A
Molecule details ›
Chain: A
Length: 455 amino acids
Theoretical weight: 48.36 KDa
Source organism: Alkalihalobacillus halodurans
Expression system: Lactococcus lactis
UniProt:
  • Canonical: Q9KDT3 (Residues: 2-453; Coverage: 100%)
Gene name: BH1128
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

5 bound ligands:
Ligand NA 2 x NA
Ligand PFF 1 x PFF
Ligand PCW 1 x PCW
Ligand LMT 6 x LMT
Ligand SOG 2 x SOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P2
Unit cell:
a: 44.15Å b: 49.85Å c: 109.73Å
α: 90° β: 96.08° γ: 90°
R-values:
R R work R free
0.225 0.223 0.261
Expression system: Lactococcus lactis

Quick links

Citations

9 citation in other articles

Structural insights into the inhibition of glycine reuptake.
Shahsavar et al. (2021)
8 more

1 mention without citation

Lactococcus lactis, an Attractive Cell Factory for the Expression of Functional Membrane Proteins.
Frelet-Barrand A. (2022)