› 6y74

X-ray diffraction
1.53Å resolution

X-ray crystal structure of human carbonic anhydrase IX catalytic domain.

Released:
DOI: 10.2210/pdb6y74/pdb
PDB entry 6y74 coloured by chain, front view.
PDB entry 6y74 coloured by chain, side view.
PDB entry 6y74 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Biophysical Characterization of Cancer-Related Carbonic Anhydrase IX
Koruza K, Murray AB, Mahon BP, Hopkins JB, Knecht W, McKenna R, Fisher SZ
Int J Mol Sci 21 5277- (2020)

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172579 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Carbonic anhydrase 9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 28.26 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q16790 (Residues: 137-394; Coverage: 61%)
Gene names: CA9, G250, MN
Sequence domains: Eukaryotic-type carbonic anhydrase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 3 x CL
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: C2
Unit cell:
a: 113.93Å b: 78.34Å c: 74.32Å
α: 90° β: 128.22° γ: 90°
R-values:
R R work R free
0.175 0.174 0.198
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017

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