› 6xt9

Electron Microscopy
3.8Å resolution

Subunits BBS 1,4,8,9,18 of the human BBSome complex

Released:
DOI: 10.2210/pdb6xt9/pdb
PDB entry 6xt9 coloured by chain, front view.
PDB entry 6xt9 coloured by chain, side view.
PDB entry 6xt9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human BBSome core complex.
Klink BU, Gatsogiannis C, Hofnagel O, Wittinghofer A, Raunser S
Elife 9 (2020)
PMID: 31951201
Related structures: EMD-10617

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-101055 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Bardet-Biedl syndrome 1 protein Chain: A
Molecule details ›
Chain: A
Length: 593 amino acids
Theoretical weight: 65.16 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q8NFJ9 (Residues: 1-593; Coverage: 100%)
Gene names: BBS1, BBS2L2
Sequence domains: Ciliary BBSome complex subunit 1
Bardet-Biedl syndrome 4 protein Chain: D
Molecule details ›
Chain: D
Length: 528 amino acids
Theoretical weight: 59.46 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q96RK4 (Residues: 1-519; Coverage: 100%)
Gene name: BBS4
Sequence domains:
Tetratricopeptide repeat domain 8 isoform 2 Chain: H
Molecule details ›
Chain: H
Length: 517 amino acids
Theoretical weight: 58.7 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A0C4DGY3 (Residues: 1-505; Coverage: 100%)
Gene name: TTC8
Sequence domains: Tetratricopeptide repeat
Protein PTHB1 Chain: I
Molecule details ›
Chain: I
Length: 887 amino acids
Theoretical weight: 99.38 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q3SYG4 (Residues: 1-887; Coverage: 100%)
Gene names: BBS9, PTHB1
Sequence domains:
BBSome-interacting protein 1 Chain: J
Molecule details ›
Chain: J
Length: 139 amino acids
Theoretical weight: 15.43 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A8MTZ0 (Residues: 1-92; Coverage: 100%)
Gene names: BBIP1, BBIP10, NCRNA00081
Sequence domains: Cilia BBSome complex subunit 10

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-10617
Expression system: Trichoplusia ni

Quick links

Citations

12 review citations

Structural insights into the architecture and assembly of eukaryotic flagella.
Petriman et al. (2020)
11 more

2 mentions without citation

Multiallelic Rare Variants in BBS Genes Support an Oligogenic Ciliopathy in a Non-obese Juvenile-Onset Syndromic Diabetic Patient: A Case Report.
Dallali et al. (2021)
1 more