› 6xqk

X-ray diffraction
2.56Å resolution

Crystal structure of the D/D domain of PKA from S. cerevisiae

Released:
DOI: 10.2210/pdb6xqk/pdb
PDB entry 6xqk coloured by chain, front view.
PDB entry 6xqk coloured by chain, side view.
PDB entry 6xqk coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The crystal structure of yeast regulatory subunit reveals key evolutionary insights into Protein Kinase A oligomerization.
Bardeci NG, Tofolón E, Trajtenberg F, Caramelo J, Larrieux N, Rossi S, Buschiazzo A, Moreno S
J Struct Biol 213 107732 (2021)
PMID: 33819633

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139475 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase regulatory subunit Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 51 amino acids
Theoretical weight: 5.9 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P07278 (Residues: 1-50; Coverage: 12%)
Gene names: BCY1, REG1, SRA1, YIL033C
Sequence domains: Regulatory subunit of type II PKA R-subunit

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 56.824Å b: 52.134Å c: 68.832Å
α: 90° β: 109.7° γ: 90°
R-values:
R R work R free
0.217 0.214 0.27
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Beyond PKA: Evolutionary and structural insights that define a docking and dimerization domain superfamily.
Dahlin et al. (2021)