› 6xkb

X-ray diffraction
1.6Å resolution

Crystal structure of SR-related and CTD-associated factor 4(SCAF4-CID)with peptide S2,S5p-CTD

Released:
DOI: 10.2210/pdb6xkb/pdb
PDB entry 6xkb coloured by chain, front view.
PDB entry 6xkb coloured by chain, side view.
PDB entry 6xkb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the recognition of the S2, S5-phosphorylated RNA polymerase II CTD by the mRNA anti-terminator protein hSCAF4.
Zhou M, Ehsan F, Gan L, Dong A, Li Y, Liu K, Min J
FEBS Lett 596 249-259 (2022)
PMID: 34897689

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131818 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SR-related and CTD-associated factor 4 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 140 amino acids
Theoretical weight: 16.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95104 (Residues: 1-139; Coverage: 12%)
Gene names: KIAA1172, SCAF4, SFRS15
Sequence domains: CID domain
DNA-directed RNA polymerase II subunit RPB1 Chains: F, G, I, J, K
Molecule details ›
Chains: F, G, I, J, K
Length: 20 amino acids
Theoretical weight: 2.53 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P24928 (Residues: 1786-1805; Coverage: 1%)
Gene names: POLR2, POLR2A
Sequence domains: RNA polymerase Rpb1 C-terminal repeat

Ligands and Environments

1 bound ligand:
Ligand UNX 12 x UNX
1 modified residue:
Ligand SEP 20 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 70.718Å b: 46.923Å c: 139.25Å
α: 90° β: 92.3° γ: 90°
R-values:
R R work R free
0.184 0.183 0.21
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

The Nrd1-Nab3-Sen1 transcription termination complex from a structural perspective.
Chaves-Arquero et al. (2023)

2 mentions without citation

What's all the phos about? Insights into the phosphorylation state of the RNA polymerase II C-terminal domain via mass spectrometry.
LeBlanc et al. (2021)
1 more